Purification:Article Title: OX40L fusion proteins and uses thereof
Article Snippet: .. TABLE 2-6 Materials Item Source AlexaFluor ® A488 goat anti-human IgG (H + L) Life Technologies, Carlsbad, CA AlexaFluor ® A488 goat anti-mouse IgG (H + L) Life Technologies, Carlsbad, CA AlexaFluor ® A488 goat anti-rat IgG (H + L) Life Technologies, Carlsbad, CA Antibiotic/antimycotic solution, 100X Life Technologies, Carlsbad, CA Anti-rat CD134 (OX40 clone) antibody Biolegend, San Jose, CA Anti-rat CD3 antibody, purified NA/LE BD Biosciences, San Jose, CA Anti-mouse CD 134 (OX86 clone) antibody MedImmune, Gaithersburg, MD Balb/C mouse Harlan, Indianapolis, IN Betamercaptoethanol Life Technologies, Carlsbad, CA Bovine serum albumin (BSA) Sigma, St Louis, MO Complete RPMI media: RPMI-1640 + 10% FBS Materials from Life Technologies, Carlsbad, CA Concanavalin A Sigma, St Louis, MO Deep well plates, polypropylene, 2 mL VWR, Radnor, PA Ethylenediaminetetraacetic acid (EDTA) Life Technologies, Carlsbad, CA Heat inactivated fetal bovine serum Life Technologies, Carlsbad, CA Hamster anti-mouse CD3 antibody, purified BD Biosciences, San Jose, CA NA/LE Hamster anti-mouse CD28 antibody, purified BD Biosciences, San Jose, CA NA/LE IL-2, recombinant human Preprotech, Rocky Hill, NJ LSR II flow cytometer BD Biosciences, San Jose, CA Lymphocyte separation media (LSM) MP Biomedicals, Santa Ana, CA Magcellect rat CD4+ T cell isolation kit R&D Systems, Minneapolis, MN Miltenyi MACS buffer Miltenyi, San Diego, CA Mouse CD4+ T cell isolation kit Miltenyi, San Diego, CA Non-human primate CD4+ T cell isolation kit Miltenyi, San Diego, CA Non-TC treated round-bottom 96 well plates VWR, Radnor, PA Nylon mesh filter, 70 micron BD Biosciences, San Jose, CA PBS, phosphate buffered saline, pH 7.2 Life Technologies, Carlsbad, CA Percoll Sigma, St. Louis, MO PHA-L Roche Applied Science, Indianapolis, IN Phosphate Buffer Saline (PBS) pH 7.2 Life Technologies, Carlsbad, CA without Calcium and Magnesium Propidium iodide (1 mg/mL solution) Sigma, Saint Louis, MO Red blood cell lysis buffer Biolegend, San Diego, CA RPMI-1640 Life Technologies, Carlsbad, CA Sprague Dawley rat Harlan, Indianapolis, IN ViCell counter Beckman Coulter, Indianapolis, IN Whole blood, sodium heparin anti-coagulated MedImmune Blood Donor Program, Gaithersburg, MD ..
Recombinant:Article Title: OX40L fusion proteins and uses thereof
Article Snippet: .. TABLE 2-6 Materials Item Source AlexaFluor ® A488 goat anti-human IgG (H + L) Life Technologies, Carlsbad, CA AlexaFluor ® A488 goat anti-mouse IgG (H + L) Life Technologies, Carlsbad, CA AlexaFluor ® A488 goat anti-rat IgG (H + L) Life Technologies, Carlsbad, CA Antibiotic/antimycotic solution, 100X Life Technologies, Carlsbad, CA Anti-rat CD134 (OX40 clone) antibody Biolegend, San Jose, CA Anti-rat CD3 antibody, purified NA/LE BD Biosciences, San Jose, CA Anti-mouse CD 134 (OX86 clone) antibody MedImmune, Gaithersburg, MD Balb/C mouse Harlan, Indianapolis, IN Betamercaptoethanol Life Technologies, Carlsbad, CA Bovine serum albumin (BSA) Sigma, St Louis, MO Complete RPMI media: RPMI-1640 + 10% FBS Materials from Life Technologies, Carlsbad, CA Concanavalin A Sigma, St Louis, MO Deep well plates, polypropylene, 2 mL VWR, Radnor, PA Ethylenediaminetetraacetic acid (EDTA) Life Technologies, Carlsbad, CA Heat inactivated fetal bovine serum Life Technologies, Carlsbad, CA Hamster anti-mouse CD3 antibody, purified BD Biosciences, San Jose, CA NA/LE Hamster anti-mouse CD28 antibody, purified BD Biosciences, San Jose, CA NA/LE IL-2, recombinant human Preprotech, Rocky Hill, NJ LSR II flow cytometer BD Biosciences, San Jose, CA Lymphocyte separation media (LSM) MP Biomedicals, Santa Ana, CA Magcellect rat CD4+ T cell isolation kit R&D Systems, Minneapolis, MN Miltenyi MACS buffer Miltenyi, San Diego, CA Mouse CD4+ T cell isolation kit Miltenyi, San Diego, CA Non-human primate CD4+ T cell isolation kit Miltenyi, San Diego, CA Non-TC treated round-bottom 96 well plates VWR, Radnor, PA Nylon mesh filter, 70 micron BD Biosciences, San Jose, CA PBS, phosphate buffered saline, pH 7.2 Life Technologies, Carlsbad, CA Percoll Sigma, St. Louis, MO PHA-L Roche Applied Science, Indianapolis, IN Phosphate Buffer Saline (PBS) pH 7.2 Life Technologies, Carlsbad, CA without Calcium and Magnesium Propidium iodide (1 mg/mL solution) Sigma, Saint Louis, MO Red blood cell lysis buffer Biolegend, San Diego, CA RPMI-1640 Life Technologies, Carlsbad, CA Sprague Dawley rat Harlan, Indianapolis, IN ViCell counter Beckman Coulter, Indianapolis, IN Whole blood, sodium heparin anti-coagulated MedImmune Blood Donor Program, Gaithersburg, MD ..
Flow Cytometry:Article Title: OX40L fusion proteins and uses thereof
Article Snippet: .. TABLE 2-6 Materials Item Source AlexaFluor ® A488 goat anti-human IgG (H + L) Life Technologies, Carlsbad, CA AlexaFluor ® A488 goat anti-mouse IgG (H + L) Life Technologies, Carlsbad, CA AlexaFluor ® A488 goat anti-rat IgG (H + L) Life Technologies, Carlsbad, CA Antibiotic/antimycotic solution, 100X Life Technologies, Carlsbad, CA Anti-rat CD134 (OX40 clone) antibody Biolegend, San Jose, CA Anti-rat CD3 antibody, purified NA/LE BD Biosciences, San Jose, CA Anti-mouse CD 134 (OX86 clone) antibody MedImmune, Gaithersburg, MD Balb/C mouse Harlan, Indianapolis, IN Betamercaptoethanol Life Technologies, Carlsbad, CA Bovine serum albumin (BSA) Sigma, St Louis, MO Complete RPMI media: RPMI-1640 + 10% FBS Materials from Life Technologies, Carlsbad, CA Concanavalin A Sigma, St Louis, MO Deep well plates, polypropylene, 2 mL VWR, Radnor, PA Ethylenediaminetetraacetic acid (EDTA) Life Technologies, Carlsbad, CA Heat inactivated fetal bovine serum Life Technologies, Carlsbad, CA Hamster anti-mouse CD3 antibody, purified BD Biosciences, San Jose, CA NA/LE Hamster anti-mouse CD28 antibody, purified BD Biosciences, San Jose, CA NA/LE IL-2, recombinant human Preprotech, Rocky Hill, NJ LSR II flow cytometer BD Biosciences, San Jose, CA Lymphocyte separation media (LSM) MP Biomedicals, Santa Ana, CA Magcellect rat CD4+ T cell isolation kit R&D Systems, Minneapolis, MN Miltenyi MACS buffer Miltenyi, San Diego, CA Mouse CD4+ T cell isolation kit Miltenyi, San Diego, CA Non-human primate CD4+ T cell isolation kit Miltenyi, San Diego, CA Non-TC treated round-bottom 96 well plates VWR, Radnor, PA Nylon mesh filter, 70 micron BD Biosciences, San Jose, CA PBS, phosphate buffered saline, pH 7.2 Life Technologies, Carlsbad, CA Percoll Sigma, St. Louis, MO PHA-L Roche Applied Science, Indianapolis, IN Phosphate Buffer Saline (PBS) pH 7.2 Life Technologies, Carlsbad, CA without Calcium and Magnesium Propidium iodide (1 mg/mL solution) Sigma, Saint Louis, MO Red blood cell lysis buffer Biolegend, San Diego, CA RPMI-1640 Life Technologies, Carlsbad, CA Sprague Dawley rat Harlan, Indianapolis, IN ViCell counter Beckman Coulter, Indianapolis, IN Whole blood, sodium heparin anti-coagulated MedImmune Blood Donor Program, Gaithersburg, MD ..
Cell Isolation:Article Title: OX40L fusion proteins and uses thereof
Article Snippet: .. TABLE 2-6 Materials Item Source AlexaFluor ® A488 goat anti-human IgG (H + L) Life Technologies, Carlsbad, CA AlexaFluor ® A488 goat anti-mouse IgG (H + L) Life Technologies, Carlsbad, CA AlexaFluor ® A488 goat anti-rat IgG (H + L) Life Technologies, Carlsbad, CA Antibiotic/antimycotic solution, 100X Life Technologies, Carlsbad, CA Anti-rat CD134 (OX40 clone) antibody Biolegend, San Jose, CA Anti-rat CD3 antibody, purified NA/LE BD Biosciences, San Jose, CA Anti-mouse CD 134 (OX86 clone) antibody MedImmune, Gaithersburg, MD Balb/C mouse Harlan, Indianapolis, IN Betamercaptoethanol Life Technologies, Carlsbad, CA Bovine serum albumin (BSA) Sigma, St Louis, MO Complete RPMI media: RPMI-1640 + 10% FBS Materials from Life Technologies, Carlsbad, CA Concanavalin A Sigma, St Louis, MO Deep well plates, polypropylene, 2 mL VWR, Radnor, PA Ethylenediaminetetraacetic acid (EDTA) Life Technologies, Carlsbad, CA Heat inactivated fetal bovine serum Life Technologies, Carlsbad, CA Hamster anti-mouse CD3 antibody, purified BD Biosciences, San Jose, CA NA/LE Hamster anti-mouse CD28 antibody, purified BD Biosciences, San Jose, CA NA/LE IL-2, recombinant human Preprotech, Rocky Hill, NJ LSR II flow cytometer BD Biosciences, San Jose, CA Lymphocyte separation media (LSM) MP Biomedicals, Santa Ana, CA Magcellect rat CD4+ T cell isolation kit R&D Systems, Minneapolis, MN Miltenyi MACS buffer Miltenyi, San Diego, CA Mouse CD4+ T cell isolation kit Miltenyi, San Diego, CA Non-human primate CD4+ T cell isolation kit Miltenyi, San Diego, CA Non-TC treated round-bottom 96 well plates VWR, Radnor, PA Nylon mesh filter, 70 micron BD Biosciences, San Jose, CA PBS, phosphate buffered saline, pH 7.2 Life Technologies, Carlsbad, CA Percoll Sigma, St. Louis, MO PHA-L Roche Applied Science, Indianapolis, IN Phosphate Buffer Saline (PBS) pH 7.2 Life Technologies, Carlsbad, CA without Calcium and Magnesium Propidium iodide (1 mg/mL solution) Sigma, Saint Louis, MO Red blood cell lysis buffer Biolegend, San Diego, CA RPMI-1640 Life Technologies, Carlsbad, CA Sprague Dawley rat Harlan, Indianapolis, IN ViCell counter Beckman Coulter, Indianapolis, IN Whole blood, sodium heparin anti-coagulated MedImmune Blood Donor Program, Gaithersburg, MD ..
Magnetic Cell Separation:Article Title: OX40L fusion proteins and uses thereof
Article Snippet: .. TABLE 2-6 Materials Item Source AlexaFluor ® A488 goat anti-human IgG (H + L) Life Technologies, Carlsbad, CA AlexaFluor ® A488 goat anti-mouse IgG (H + L) Life Technologies, Carlsbad, CA AlexaFluor ® A488 goat anti-rat IgG (H + L) Life Technologies, Carlsbad, CA Antibiotic/antimycotic solution, 100X Life Technologies, Carlsbad, CA Anti-rat CD134 (OX40 clone) antibody Biolegend, San Jose, CA Anti-rat CD3 antibody, purified NA/LE BD Biosciences, San Jose, CA Anti-mouse CD 134 (OX86 clone) antibody MedImmune, Gaithersburg, MD Balb/C mouse Harlan, Indianapolis, IN Betamercaptoethanol Life Technologies, Carlsbad, CA Bovine serum albumin (BSA) Sigma, St Louis, MO Complete RPMI media: RPMI-1640 + 10% FBS Materials from Life Technologies, Carlsbad, CA Concanavalin A Sigma, St Louis, MO Deep well plates, polypropylene, 2 mL VWR, Radnor, PA Ethylenediaminetetraacetic acid (EDTA) Life Technologies, Carlsbad, CA Heat inactivated fetal bovine serum Life Technologies, Carlsbad, CA Hamster anti-mouse CD3 antibody, purified BD Biosciences, San Jose, CA NA/LE Hamster anti-mouse CD28 antibody, purified BD Biosciences, San Jose, CA NA/LE IL-2, recombinant human Preprotech, Rocky Hill, NJ LSR II flow cytometer BD Biosciences, San Jose, CA Lymphocyte separation media (LSM) MP Biomedicals, Santa Ana, CA Magcellect rat CD4+ T cell isolation kit R&D Systems, Minneapolis, MN Miltenyi MACS buffer Miltenyi, San Diego, CA Mouse CD4+ T cell isolation kit Miltenyi, San Diego, CA Non-human primate CD4+ T cell isolation kit Miltenyi, San Diego, CA Non-TC treated round-bottom 96 well plates VWR, Radnor, PA Nylon mesh filter, 70 micron BD Biosciences, San Jose, CA PBS, phosphate buffered saline, pH 7.2 Life Technologies, Carlsbad, CA Percoll Sigma, St. Louis, MO PHA-L Roche Applied Science, Indianapolis, IN Phosphate Buffer Saline (PBS) pH 7.2 Life Technologies, Carlsbad, CA without Calcium and Magnesium Propidium iodide (1 mg/mL solution) Sigma, Saint Louis, MO Red blood cell lysis buffer Biolegend, San Diego, CA RPMI-1640 Life Technologies, Carlsbad, CA Sprague Dawley rat Harlan, Indianapolis, IN ViCell counter Beckman Coulter, Indianapolis, IN Whole blood, sodium heparin anti-coagulated MedImmune Blood Donor Program, Gaithersburg, MD ..
Saline:Article Title: OX40L fusion proteins and uses thereof
Article Snippet: .. TABLE 2-6 Materials Item Source AlexaFluor ® A488 goat anti-human IgG (H + L) Life Technologies, Carlsbad, CA AlexaFluor ® A488 goat anti-mouse IgG (H + L) Life Technologies, Carlsbad, CA AlexaFluor ® A488 goat anti-rat IgG (H + L) Life Technologies, Carlsbad, CA Antibiotic/antimycotic solution, 100X Life Technologies, Carlsbad, CA Anti-rat CD134 (OX40 clone) antibody Biolegend, San Jose, CA Anti-rat CD3 antibody, purified NA/LE BD Biosciences, San Jose, CA Anti-mouse CD 134 (OX86 clone) antibody MedImmune, Gaithersburg, MD Balb/C mouse Harlan, Indianapolis, IN Betamercaptoethanol Life Technologies, Carlsbad, CA Bovine serum albumin (BSA) Sigma, St Louis, MO Complete RPMI media: RPMI-1640 + 10% FBS Materials from Life Technologies, Carlsbad, CA Concanavalin A Sigma, St Louis, MO Deep well plates, polypropylene, 2 mL VWR, Radnor, PA Ethylenediaminetetraacetic acid (EDTA) Life Technologies, Carlsbad, CA Heat inactivated fetal bovine serum Life Technologies, Carlsbad, CA Hamster anti-mouse CD3 antibody, purified BD Biosciences, San Jose, CA NA/LE Hamster anti-mouse CD28 antibody, purified BD Biosciences, San Jose, CA NA/LE IL-2, recombinant human Preprotech, Rocky Hill, NJ LSR II flow cytometer BD Biosciences, San Jose, CA Lymphocyte separation media (LSM) MP Biomedicals, Santa Ana, CA Magcellect rat CD4+ T cell isolation kit R&D Systems, Minneapolis, MN Miltenyi MACS buffer Miltenyi, San Diego, CA Mouse CD4+ T cell isolation kit Miltenyi, San Diego, CA Non-human primate CD4+ T cell isolation kit Miltenyi, San Diego, CA Non-TC treated round-bottom 96 well plates VWR, Radnor, PA Nylon mesh filter, 70 micron BD Biosciences, San Jose, CA PBS, phosphate buffered saline, pH 7.2 Life Technologies, Carlsbad, CA Percoll Sigma, St. Louis, MO PHA-L Roche Applied Science, Indianapolis, IN Phosphate Buffer Saline (PBS) pH 7.2 Life Technologies, Carlsbad, CA without Calcium and Magnesium Propidium iodide (1 mg/mL solution) Sigma, Saint Louis, MO Red blood cell lysis buffer Biolegend, San Diego, CA RPMI-1640 Life Technologies, Carlsbad, CA Sprague Dawley rat Harlan, Indianapolis, IN ViCell counter Beckman Coulter, Indianapolis, IN Whole blood, sodium heparin anti-coagulated MedImmune Blood Donor Program, Gaithersburg, MD ..
Red Blood Cell Lysis:Article Title: OX40L fusion proteins and uses thereof
Article Snippet: .. TABLE 2-6 Materials Item Source AlexaFluor ® A488 goat anti-human IgG (H + L) Life Technologies, Carlsbad, CA AlexaFluor ® A488 goat anti-mouse IgG (H + L) Life Technologies, Carlsbad, CA AlexaFluor ® A488 goat anti-rat IgG (H + L) Life Technologies, Carlsbad, CA Antibiotic/antimycotic solution, 100X Life Technologies, Carlsbad, CA Anti-rat CD134 (OX40 clone) antibody Biolegend, San Jose, CA Anti-rat CD3 antibody, purified NA/LE BD Biosciences, San Jose, CA Anti-mouse CD 134 (OX86 clone) antibody MedImmune, Gaithersburg, MD Balb/C mouse Harlan, Indianapolis, IN Betamercaptoethanol Life Technologies, Carlsbad, CA Bovine serum albumin (BSA) Sigma, St Louis, MO Complete RPMI media: RPMI-1640 + 10% FBS Materials from Life Technologies, Carlsbad, CA Concanavalin A Sigma, St Louis, MO Deep well plates, polypropylene, 2 mL VWR, Radnor, PA Ethylenediaminetetraacetic acid (EDTA) Life Technologies, Carlsbad, CA Heat inactivated fetal bovine serum Life Technologies, Carlsbad, CA Hamster anti-mouse CD3 antibody, purified BD Biosciences, San Jose, CA NA/LE Hamster anti-mouse CD28 antibody, purified BD Biosciences, San Jose, CA NA/LE IL-2, recombinant human Preprotech, Rocky Hill, NJ LSR II flow cytometer BD Biosciences, San Jose, CA Lymphocyte separation media (LSM) MP Biomedicals, Santa Ana, CA Magcellect rat CD4+ T cell isolation kit R&D Systems, Minneapolis, MN Miltenyi MACS buffer Miltenyi, San Diego, CA Mouse CD4+ T cell isolation kit Miltenyi, San Diego, CA Non-human primate CD4+ T cell isolation kit Miltenyi, San Diego, CA Non-TC treated round-bottom 96 well plates VWR, Radnor, PA Nylon mesh filter, 70 micron BD Biosciences, San Jose, CA PBS, phosphate buffered saline, pH 7.2 Life Technologies, Carlsbad, CA Percoll Sigma, St. Louis, MO PHA-L Roche Applied Science, Indianapolis, IN Phosphate Buffer Saline (PBS) pH 7.2 Life Technologies, Carlsbad, CA without Calcium and Magnesium Propidium iodide (1 mg/mL solution) Sigma, Saint Louis, MO Red blood cell lysis buffer Biolegend, San Diego, CA RPMI-1640 Life Technologies, Carlsbad, CA Sprague Dawley rat Harlan, Indianapolis, IN ViCell counter Beckman Coulter, Indianapolis, IN Whole blood, sodium heparin anti-coagulated MedImmune Blood Donor Program, Gaithersburg, MD ..
Suspension:Article Title: Alternation in colonization behaviors of Escherichia coli cells with rpoS or yggE deficiency on solid surfaces.
Article Snippet: Colonization on a solid surface is influenced by the cell surface appendages such as flagella and curli, of which expressions are regulated by rpoS gene encoding a sigma factor.. In this study, we investigated the effect of rpoS or yggE (a rpoS-related and stress-responsive gene) deficiency on the colonization of Escherichia coli BW25113.. Under a static condition, the deletion of rpoS or yggE induced 3.9and 3.7-fold higher colonization as compared to wild-type cells, respectively, on the solid surfaces.
Clinical Proteomics:Article Title: Development of Immunoassays for the Quantitative Assessment of Amyloid-β in the Presence of Therapeutic Antibody: Application to Pre-Clinical Studies
Article Snippet: .. Plasma was transferred into pre-chilled Matrix tubes, 1.4 mL polypropylene (Thermo Scientific 4140) and the plasma sample aliquots were snap-frozen on dry ice and stored immediately at −70 to −90◦C pending analysis. ..
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